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nexttec™ DNA isolation
system
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The nexttec™ DNA
isolation system (nexttec™ cleanColumns and nexttec™ cleanPlates96) allows
to get pure DNA from a wide range of sample materials (tissue, cells, plant,
blood, plasmid DNA or genomic DNA from bacteria) in only 4 minutes* using one
step for purification. *after lysis
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Principle
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The nexttec™ DNA
Purification is a reversal of the commonly used silica-based systems.
Proteins and other inhibiting substances are retained on
the surface of a specially
developed sorbent. DNA is NOT bound and passes
through the sorbent layer.
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| Main advanteges of this worldwide unique system: |
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- time saving (up to 5 times faster* than
the market leader, figure 1)
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- only one centrifugation step at 700x g
for purification (nexttec™ cleanColumns and nexttec™
cleanPlates96 )
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- nearly loss-free DNA preparation down to
2 pg DNA (70% -80% recovery, figure 2)
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- waste reduction (minimal disposables,
tips and chemicals)
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- ready-to-use kits without hazardous
chemicals
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- no expensive equipment needed for
high through-put application
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Figure 1: Time necessary for DNA
Purification
using the nexttec™ DNA isolation system
compared to silica-based systems.
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Figure 2: Purification of DNA in the
picogram
range using the nexttec™ DNA
isolation
system and a silica-based system.
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Principles 